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- GRAPHPAD PRISM 8 STEP BY STEP EXAMPLE HOW TO
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The demonstrations will indicate how to get started with the Prism software. In this article, the basic interface of the GraphPad Prism is demonstrated. normalized response - Variable slope Dose-response - Special Asymmetrical (five. normalized response Equation: log(inhibitor). response - Variable slope Equation: log(inhibitor). normalized response - Variable slope Dose-response - Inhibition Equation: log(inhibitor). normalized response Equation: log(agonist). response - Variable slope Equation: log(agonist). to log(concentration) Dose-response - Stimulation Equation: log(agonist). 112 Nonlinear regression with Prism Choosing a built-in model Dose-response - Key concepts
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82 Nonlinear regression tutorials Example: Fitting an enzyme kinetics curve Example: Comparing two enzyme kinetics models Example: Interpolating from a sigmoidal standard curve Example: Automatic outlier elimination (exponential decay) Example: Global nonlinear regression (dose-response curves) Example: Ambiguous fit (dose-response) 51 Key concepts in nonlinear regression Introducing nonlinear regression The goal of nonlinear regression The differences between linear and nonlinear regression Distinguishing nonlinear regression from other kinds of regression Preparing data for nonlinear regression Understanding models What is a model? Three example models Why can't GraphPad Prism choose a model? Advice: How to understand a model Comparing models Questions that can be answered by comparing models Approaches to comparing models How the F test works to compare models How the AICc computations work Global nonlinear regression What is global nonlinear regression? Using global regression to fit incomplete datasets Fitting models where the parameters are defined by multiple data sets Column constants Advice: Don't use global regression if datasets use different units Outlier elimination and robust nonlinear regression When to use automatic outlier removal When to avoid automatic outlier removal Outliers aren't always 'bad' points The ROUT method of identifying outliers Robust nonlinear regression How nonlinear regression works Why minimize the sum-of-squares? How nonlinear regression works Unequal weighting in nonlinear regression How standard errors and confidence intervals are computed How confidence and prediction bands are computed Replicates How dependency is calculated Key concepts: Deming regression How to: Deming regression Analysis checklist: Deming regression 39 Results of linear regression Slope and intercept r2, a measure of goodness-of-fit of linear regression Is the slope significantly different than zero? Comparing slopes and intercepts Runs test following linear regression Analysis checklist: Linear regression Graphing tips: Linear regression 36 regression Finding the best-fit slope and intercept Interpolating from a linear standard curve 32 Key concepts: Linear regression The goal of linear regression How linear regression works Advice: Avoid Scatchard, Lineweaver-Burke and similar transforms Advice: When to fit a line with nonlinear regression 29 Simulations and script to assess confidence intervals 28 Using a script to simulate many data sets. 21 Plotting a function Simulating data.23 with random error. 17 Spline and Lowess curves Using nonlinear.19 regression with an empirical model
GRAPHPAD PRISM 8 STEP BY STEP EXAMPLE SOFTWARE
Motulsky, Prism 5 Statistics Guide, 2007, GraphPad Software Inc., San Diego CA, To contact GraphPad Software, email or Ĭontents I. GraphPad is a trademark of GraphPad Software, Inc. GraphPad Prism and Prism are registered trademarks of GraphPad Software, Inc. You may also view the Prism Help on the web at: Ĭopyright 2007, GraphPad Software, Inc.
GRAPHPAD PRISM 8 STEP BY STEP EXAMPLE FREE
The companion Statistics Guide explains how to do statistical analyses with Prism, Both of these Guides contain exactly the same information as the Help system that comes with Prism 5, including the free demo versrion. Only about 10% of this Guide is specific to Prism, so you may find it very useful even if you use another regression program.
GRAPHPAD PRISM 8 STEP BY STEP EXAMPLE DOWNLOAD
Download a free demo from The focus of this Guide is on helping you understand the big ideas behind regression so you can choose an analysis and interpret the results.
GRAPHPAD PRISM 8 STEP BY STEP EXAMPLE MAC
Available for both Mac and Windows, Prism makes it very easy to graph and analyze scientific data. This Regression Guide is a companion to GraphPad Prism 5. Harvey Motulsky President, GraphPad Software Inc.